Part:BBa_K249026:Experience

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BBa_K249026 Experience by Wits-CSIR_SA iGEM2011

Team Lethbridge 2009, used the Gallivan theophylline riboswitch (clone 8.1) to generate a riboswitch-CheZ construct. However, this team created a 11 nt insert after the ATG start codon. This result created a TAG stop codon (in the 3rd amino acid position) as well as a frameshift mutation. Therefore it is unlikely that a functional CheZ could have been produced from this system. (see team Lethbridge 2009 below, riboswitch highlighted in yellow, inserted biobrick “scar” region highlighted in violet, ATG start underlined) BBa_K249026 - Team Lethbridge iGEM2009 For more detailed analysis of how this part can be improved, visit our results page[http://2011.igem.org/Team:WITS-CSIR_SA/Project/Concept] and the following parts:BBa_K537001 BBa_K537003

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